The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Identifieur interne : 002152 ( Main/Exploration ); précédent : 002151; suivant : 002153The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Auteurs : Mugurel Constantin Rusu [Roumanie] ; Andreea Cristiana Didilescu ; Ruxandra St Nescu ; Florinel Pop ; Valentina Mariana M Noiu ; Adelina Maria Jianu ; Marek VâlcuSource :
- Anatomical record (Hoboken, N.J. : 2007) [ 1932-8494 ] ; 2013.
Descripteurs français
- KwdFr :
- Adulte, Cellules endothéliales (), Cellules endothéliales (ultrastructure), Cellules stromales (), Cellules stromales (ultrastructure), Cicatrisation de plaie, Femelle, Humains, Immunohistochimie, Mandibule (), Mandibule (ultrastructure), Marqueurs biologiques (analyse), Microscopie électronique à transmission, Muqueuse de la bouche (), Muqueuse de la bouche (ultrastructure), Mâchoire édentée (), Mâchoire édentée (anatomopathologie), Mâchoire édentée (métabolisme), Mâle, Néovascularisation physiologique, Phénotype, Pose d'implant dentaire, Études cas-témoins.
- MESH :
- analyse : Marqueurs biologiques.
- anatomopathologie : Mâchoire édentée.
- métabolisme : Mâchoire édentée.
- Adulte, Cellules endothéliales, Cellules stromales, Cicatrisation de plaie, Femelle, Humains, Immunohistochimie, Mandibule, Microscopie électronique à transmission, Muqueuse de la bouche, Mâchoire édentée, Mâle, Néovascularisation physiologique, Phénotype, Pose d'implant dentaire, Études cas-témoins.
English descriptors
- KwdEn :
- Adult, Biomarkers (analysis), Case-Control Studies, Dental Implantation, Endothelial Cells (chemistry), Endothelial Cells (ultrastructure), Female, Humans, Immunohistochemistry, Jaw, Edentulous (metabolism), Jaw, Edentulous (pathology), Jaw, Edentulous (surgery), Male, Mandible (blood supply), Mandible (chemistry), Mandible (ultrastructure), Microscopy, Electron, Transmission, Mouth Mucosa (blood supply), Mouth Mucosa (chemistry), Mouth Mucosa (ultrastructure), Neovascularization, Physiologic, Phenotype, Stromal Cells (chemistry), Stromal Cells (ultrastructure), Wound Healing.
- MESH :
- chemical , analysis : Biomarkers.
- blood supply : Mandible, Mouth Mucosa.
- chemistry : Endothelial Cells, Mandible, Mouth Mucosa, Stromal Cells.
- metabolism : Jaw, Edentulous.
- pathology : Jaw, Edentulous.
- surgery : Jaw, Edentulous.
- ultrastructure : Endothelial Cells, Mandible, Mouth Mucosa, Stromal Cells.
- Adult, Case-Control Studies, Dental Implantation, Female, Humans, Immunohistochemistry, Male, Microscopy, Electron, Transmission, Neovascularization, Physiologic, Phenotype, Wound Healing.
Abstract
This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-α), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/CD44/CD45/CD105/PDGFR-α/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral mucosa are so supported by extensive processes of angiogenesis, guided by ETCs that, in turn, are influenced by the CFCs that populate the stromal compartment both in normal and reparatory states. Therefore, CFCs could be targeted by specific therapies, with pro- or anti-angiogenic purposes.
DOI: 10.1002/ar.22630
PubMed: 23192856
Affiliations:
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sort="Rusu, Mugurel Constantin" uniqKey="Rusu M" first="Mugurel Constantin" last="Rusu">Mugurel Constantin Rusu</name><affiliation wicri:level="1"><nlm:affiliation>Division of Anatomy, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. anatomon@gmail.com</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Division of Anatomy, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest</wicri:regionArea><wicri:noRegion>Bucharest</wicri:noRegion></affiliation></author><author><name sortKey="Didilescu, Andreea Cristiana" sort="Didilescu, Andreea Cristiana" uniqKey="Didilescu A" first="Andreea Cristiana" last="Didilescu">Andreea Cristiana Didilescu</name></author><author><name sortKey="St Nescu, Ruxandra" sort="St Nescu, Ruxandra" uniqKey="St Nescu R" first="Ruxandra" last="St Nescu">Ruxandra St Nescu</name></author><author><name sortKey="Pop, Florinel" sort="Pop, Florinel" uniqKey="Pop F" first="Florinel" last="Pop">Florinel Pop</name></author><author><name sortKey="M Noiu, Valentina Mariana" sort="M Noiu, Valentina Mariana" uniqKey="M Noiu V" first="Valentina Mariana" last="M Noiu">Valentina Mariana M Noiu</name></author><author><name sortKey="Jianu, Adelina Maria" sort="Jianu, Adelina Maria" uniqKey="Jianu A" first="Adelina Maria" last="Jianu">Adelina Maria Jianu</name></author><author><name sortKey="Valcu, Marek" sort="Valcu, Marek" uniqKey="Valcu M" first="Marek" last="Vâlcu">Marek Vâlcu</name></author></analytic><series><title level="j">Anatomical record (Hoboken, N.J. : 2007)</title><idno type="eISSN">1932-8494</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Biomarkers (analysis)</term><term>Case-Control Studies</term><term>Dental Implantation</term><term>Endothelial Cells (chemistry)</term><term>Endothelial Cells (ultrastructure)</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Jaw, Edentulous (metabolism)</term><term>Jaw, Edentulous (pathology)</term><term>Jaw, Edentulous (surgery)</term><term>Male</term><term>Mandible (blood supply)</term><term>Mandible (chemistry)</term><term>Mandible (ultrastructure)</term><term>Microscopy, Electron, Transmission</term><term>Mouth Mucosa (blood supply)</term><term>Mouth Mucosa (chemistry)</term><term>Mouth Mucosa (ultrastructure)</term><term>Neovascularization, Physiologic</term><term>Phenotype</term><term>Stromal Cells (chemistry)</term><term>Stromal Cells (ultrastructure)</term><term>Wound Healing</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Cellules endothéliales ()</term><term>Cellules endothéliales (ultrastructure)</term><term>Cellules stromales ()</term><term>Cellules stromales (ultrastructure)</term><term>Cicatrisation de plaie</term><term>Femelle</term><term>Humains</term><term>Immunohistochimie</term><term>Mandibule ()</term><term>Mandibule (ultrastructure)</term><term>Marqueurs biologiques (analyse)</term><term>Microscopie électronique à transmission</term><term>Muqueuse de la bouche ()</term><term>Muqueuse de la bouche (ultrastructure)</term><term>Mâchoire édentée ()</term><term>Mâchoire édentée (anatomopathologie)</term><term>Mâchoire édentée (métabolisme)</term><term>Mâle</term><term>Néovascularisation physiologique</term><term>Phénotype</term><term>Pose d'implant dentaire</term><term>Études cas-témoins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Mâchoire édentée</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Mandible</term><term>Mouth Mucosa</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Endothelial Cells</term><term>Mandible</term><term>Mouth Mucosa</term><term>Stromal Cells</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mâchoire édentée</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Endothelial Cells</term><term>Mandible</term><term>Mouth Mucosa</term><term>Stromal Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Case-Control Studies</term><term>Dental Implantation</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Male</term><term>Microscopy, Electron, Transmission</term><term>Neovascularization, Physiologic</term><term>Phenotype</term><term>Wound Healing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Cellules endothéliales</term><term>Cellules stromales</term><term>Cicatrisation de plaie</term><term>Femelle</term><term>Humains</term><term>Immunohistochimie</term><term>Mandibule</term><term>Microscopie électronique à transmission</term><term>Muqueuse de la bouche</term><term>Mâchoire édentée</term><term>Mâle</term><term>Néovascularisation physiologique</term><term>Phénotype</term><term>Pose d'implant dentaire</term><term>Études cas-témoins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-α), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/CD44/CD45/CD105/PDGFR-α/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral mucosa are so supported by extensive processes of angiogenesis, guided by ETCs that, in turn, are influenced by the CFCs that populate the stromal compartment both in normal and reparatory states. Therefore, CFCs could be targeted by specific therapies, with pro- or anti-angiogenic purposes.</div></front></TEI><affiliations><list><country><li>Roumanie</li></country></list><tree><noCountry><name sortKey="Didilescu, Andreea Cristiana" sort="Didilescu, Andreea Cristiana" uniqKey="Didilescu A" first="Andreea Cristiana" last="Didilescu">Andreea Cristiana Didilescu</name><name sortKey="Jianu, Adelina Maria" sort="Jianu, Adelina Maria" uniqKey="Jianu A" first="Adelina Maria" last="Jianu">Adelina Maria Jianu</name><name sortKey="M Noiu, Valentina Mariana" sort="M Noiu, Valentina Mariana" uniqKey="M Noiu V" first="Valentina Mariana" last="M Noiu">Valentina Mariana M Noiu</name><name sortKey="Pop, Florinel" sort="Pop, Florinel" uniqKey="Pop F" first="Florinel" last="Pop">Florinel Pop</name><name sortKey="St Nescu, Ruxandra" sort="St Nescu, Ruxandra" uniqKey="St Nescu R" first="Ruxandra" last="St Nescu">Ruxandra St Nescu</name><name sortKey="Valcu, Marek" sort="Valcu, Marek" uniqKey="Valcu M" first="Marek" last="Vâlcu">Marek Vâlcu</name></noCountry><country name="Roumanie"><noRegion><name sortKey="Rusu, Mugurel Constantin" sort="Rusu, Mugurel Constantin" uniqKey="Rusu M" first="Mugurel Constantin" last="Rusu">Mugurel Constantin Rusu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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